Product Description
2X RT-PCR Master Mix is used for real-time qualitative and quantitative multiplex RT-PCR amplifications with TaqMan probes.
The kit is designed for multiplex RT-PCR with up to five pairs of primers and five TaqMan probes.
The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and RNA templates.
In a single-tube format, multiplex RNA templates are first converted to cDNAs by a reverse transcriptase, then a DNA-dependent DNA polymerase amplifies the cDNAs and its 5´-3´exonuclease cleaves TaqMan probes to generate fluorescent signals.
For the reverse transcription step, this kit uses a super-efficient Thermophilic Reverse Transcriptase (US patent pending), a thermophilic type A polymerase, which works best at 60-62°C, overcoming difficulties due to high GC content and abundant secondary structure. The RTase efficiently synthesizes a complementary DNA strands on RNA templates with 1-2 unit per 20μL of reaction. The RTase reversely-transcribes single digit copies of target RNA molecules consistently.
The kit also contains qPCR polymerase, which is specially engineered for TaqMan probes and its increased 5’-3’ exonuclease activity generating S-shaped curves.
Characteristics
• For the reverse transcription step, this kit uses a super-efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.
• The RTase is easily heat-inactivated at ≥90°C for 1min.
• The RTase efficiently synthesizes complementary DNA strands on RNA templates from agene-specific primers, 1-2 unit per 20μL of reaction.
• The RTase reversely-transcribes single digit copies of target RNA molecules consistently.
• The kit also contains Taq-Probe DNA polymerase specially engineered for TaqMan probes, generating S-shaped curves.
• The preferable PCR product sizes are ≤150bp.
Catalogue
|
NO. |
Item |
Product Name |
Specification |
Concentration |
|
1 |
Cat.20230227 |
DNA Polymerase |
2500U/Bottle |
25U/μL |
|
2 |
Cat.20230228 |
DNA Polymerase |
2500U/Bottle |
25U/μL |
|
3 |
Cat.20230215 |
5X STR Buffer |
5mL/Bottle |
- |
|
4 |
Cat.20230110 |
2X qPCR Master Mix |
1mL/Bottle |
- |
|
5 |
Cat.20230115 |
2X RT-PCR Master Mix |
1mL/Bottle |
- |
|
6 |
Cat.20230226 |
qPCR Polymerase |
2500U/Bottle |
25U/μL |
|
7 |
Cat.20230301 |
Thermophilic Reverse Transcriptase |
500U/Bottle |
10U/μL |
|
8 |
Cat.20230210 |
5X PCR Buffer |
5mL/Bottle |
- |